Basic Vector Information
- Vector Name:
- Beta-arrestin2 GFP WT
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5954 bp
- Type:
- Mammalian Expression
- Replication origin:
- ori
- Copy Number:
- High Copy
- Promoter:
- CMV
- Cloning Method:
- Restriction Enzyme
- 3' Primer:
- EGFP-N
Beta-arrestin2 GFP WT vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Beta-arrestin2 GFP WT vector Sequence
LOCUS V000378 5954 bp DNA circular SYN 13-MAY-2021
DEFINITION Exported.
ACCESSION V000378
VERSION V000378
KEYWORDS Beta-arrestin2 GFP WT.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 5954)
AUTHORS Shenoy SK, Lefkowitz RJ
TITLE Receptor-specific ubiquitination of beta-arrestin directs assembly
and targeting of seven-transmembrane receptor signalosomes.
JOURNAL J Biol Chem. 2005 Apr 15;280(15):15315-24. Epub 2005 Feb 7.
PUBMED 15699045
REFERENCE 2 (bases 1 to 5954)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 5954)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J Biol
Chem."; date: "2005-04-15"; volume: "280(15)"; pages: "15315-24.
Epub 2005 Feb 7"
SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5954
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 1..304
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 583..1812
/gene="Arrb2"
/label="Beta-arrestin-2"
/note="Beta-arrestin-2 from Rattus norvegicus. Accession#:
P29067"
CDS 1840..2556
/label="EGFP"
/note="enhanced GFP"
polyA_signal 2682..2803
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2810..3265)
/direction=LEFT
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3292..3396
/label="AmpR promoter"
promoter 3398..3755
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 3790..4581
/label="NeoR/KanR"
/note="aminoglycoside phosphotransferase"
primer_bind complement(4772..4791)
/label="TK-pA-R"
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 4816..4863
/label="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5192..5780
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 5895..5954
/label="CMV"
/note="Human cytomegalovirus immediate early
enhancer/promoter"
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