Basic Vector Information
- Vector Name:
- pVITRO1-SS-113
- Antibiotic Resistance:
- Kanamycin
- Length:
- 7477 bp
- Type:
- Protein expression
- Replication origin:
- ori
- Host:
- Mammalian cells
- Selection Marker:
- Neo/G418
- Promoter:
- mEF-1α
- Growth Strain(s):
- DH5a
- Growth Temperature:
- 37℃
pVITRO1-SS-113 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVITRO1-SS-113 vector Sequence
LOCUS 62056_22585 7477 bp DNA circular SYN 01-JAN-1980
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7477)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..7477
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 51..242
/label=SV40 enhancer
/note="enhancer for the SV40 early promoter (Herr, 1993)"
promoter 256..1562
/label=mEF-1-alpha promoter
/note="strong constitutive promoter for mouse elongation
factor EF-1-alpha"
CDS 1585..1608
/codon_start=1
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/translation="DYKDDDDK"
CDS 1609..1629
/codon_start=1
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/translation="PKKKRKV"
CDS 1909..2058
/codon_start=1
/label=VP64
/note="tetrameric repeat of the minimal activation domain
of herpes simplex virus VP16 (Beerli et al., 1998)"
/translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
DLDML"
polyA_signal complement(2090..2211)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 2399..2987
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 3067..3370
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3485..4790
/label=rEF-1-alpha promoter
/note="strong constitutive promoter for rat elongation
factor EF-1-alpha"
CDS 4803..5510
/codon_start=1
/label=mCherry
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWQASSERMYPEDGALK
GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
EGRHSTGGMDELYK"
misc_feature 5520..5964
/label=FMDV IRES
/note="internal ribosome entry site (IRES) of the
foot-and-mouth disease virus"
promoter 5996..6043
/label=EM7 promoter
/note="synthetic bacterial promoter"
CDS 6063..6854
/codon_start=1
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 6904..7476
/label=EF-1-alpha poly(A) signal
/note="human EF-1-alpha polyadenylation signal"
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