Basic Vector Information
- Vector Name:
- ARR3tk-eGFP/SV40-mCherry
- Antibiotic Resistance:
- Ampicillin
- Length:
- 8622 bp
- Type:
- Transcription factor reporter
- Replication origin:
- ori
- Host:
- Mammalian cells, Lentivirus
- Promoter:
- RSV
- Growth Strain(s):
- Stbl3
- Growth Temperature:
- 37℃
ARR3tk-eGFP/SV40-mCherry vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
ARR3tk-eGFP/SV40-mCherry vector Sequence
LOCUS V017128 8622 bp DNA circular SYN 01-JAN-1980
DEFINITION Exported.
ACCESSION V017128
VERSION V017128
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 8622)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8622
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 232..253
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 268..298
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 306..322
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 330..346
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 367..385
/label="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 413..639
/label="RSV promoter"
/note="Rous sarcoma virus enhancer/promoter"
misc_feature 867..992
/label="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1485..1718
/label="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1903..1947
/label="gp41 peptide"
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 2096..2137
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
/label="Protein Tat"
misc_feature 2214..2331
/label="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2888..2919
/label="minP"
/note="minimal TATA-box promoter with low basal activity"
CDS 2981..3697
/label="EGFP"
/note="enhanced GFP"
promoter 3794..4151
/label="SV40 promoter"
/note="SV40 enhancer and early promoter"
CDS 4161..4868
/label="mCherry"
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
misc_feature 4878..5466
/label="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 5553..5786
/label="3' LTR (Delta-U3)"
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 5858..5992
/label="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 6019..6154
/label="SV40 ori"
/note="SV40 origin of replication"
promoter complement(6175..6193)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6203..6219)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 6361..6816
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6842..6946
/label="AmpR promoter"
CDS 6947..7804
/label="AmpR"
/note="beta-lactamase"
rep_origin 7978..8566
/direction=RIGHT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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