Basic Vector Information
- Vector Name:
- 3XFlag-SMXL6
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7307 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Host:
- Plants
- Source/Author:
- Wang L.
- Promoter:
- CaMV 35S
3XFlag-SMXL6 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
3XFlag-SMXL6 vector Sequence
LOCUS V015409 7307 bp DNA circular SYN 20-JAN-2020
DEFINITION Exported.
ACCESSION V015409
VERSION V015409
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 7307)
AUTHORS Wang L.
TITLE Direct Submission
JOURNAL Submitted (22-NOV-2019) State Key Laboratory of Plant Genomics,
Institute of Genetics and Developmental Biology, CAS, West Beichen
Road, Bei Jing 100101, China
REFERENCE 2 (bases 1 to 7307)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
(22-NOV-2019) State Key Laboratory of Plant Genomics, Institute of
Genetics and Developmental Biology, CAS, West Beichen Road, Bei Jing
100101, China"
FEATURES Location/Qualifiers
source 1..7307
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..345
/label="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 448..513
/label="3xFLAG"
/note="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
protein_bind 514..538
/label="attB1"
/note="recombination site for the Gateway(R) BP reaction"
CDS 541..3477
/gene="SMXL6"
/label="Protein SMAX1-LIKE 6"
/note="Protein SMAX1-LIKE 6 from Arabidopsis thaliana.
Accession#: Q9LML2"
protein_bind complement(3482..3506)
/label="attB2"
/note="recombination site for the Gateway(R) BP reaction"
promoter complement(3771..3789)
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3796..3812)
/label="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 3953..4408
/label="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4486..4590
/label="AmpR promoter"
CDS 4591..5448
/label="AmpR"
/note="beta-lactamase"
rep_origin 5622..6210
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 6498..6519
/label="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6534..6564
/label="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 6572..6588
/label="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6596..6612
/label="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
promoter 6630..6648
/label="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
promoter 6821..7307
/label="CaMV35S(long)"
/note="Cauliflower mosaic virus 35S promoter (long)"
This page is informational only.