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pMO746 vector (V014686)

Basic Vector Information

Vector Name:
pMO746
Antibiotic Resistance:
Ampicillin, Kanamycin
Length:
3206 bp
Type:
Gene cloning
Replication origin:
ori
Host:
E. coli
Growth Temperature:
37℃

pMO746 vector Vector Map

pMO7463206 bp6001200180024003000AmpRoriUracil phosphoribosyltransferaseNeoR/KanR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMO746 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V014686                 3206 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014686
VERSION     V014686
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..3206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(5..862)
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      986..1574
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1638..2261)
                     /gene="upp"
                     /label="Uracil phosphoribosyltransferase"
                     /note="Uracil phosphoribosyltransferase from Desulfovibrio
                     vulgaris subsp. vulgaris (strain DP4). Accession#: A1VEW8"
     CDS             complement(2277..3068)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"

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