Basic Vector Information
- Vector Name:
- pMAL-p5E
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5755 bp
- Type:
- Cloning Vectors
- Replication origin:
- ori
- Source/Author:
- Riggs P.
- Copy Number:
- High copy number
pMAL-p5E vector Map
pMAL-p5E vector Sequence
LOCUS pMAL-p5E. 5755 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for inducible periplasmic expression of maltose-binding protein (MBP) fusions with an enterokinase cleavage site. ACCESSION . VERSION . KEYWORDS pMAL-p5E SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5755) AUTHORS Riggs P. TITLE Expression and purification of maltose-binding protein fusions. JOURNAL Curr Protoc Mol Biol 2001;Chapter 16:Unit16.6. PUBMED 18265133 REFERENCE 2 (bases 1 to 5755) AUTHORS New England Biolabs TITLE Direct Submission REFERENCE 3 (bases 1 to 5755) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc Mol Biol"; date: "2001"; volume: "Chapter 16"; pages: "Unit16.6" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..5755 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 3..80 /label=lacIq promoter /note="In the lacIq allele, a single base change in the promoter boosts expression of the lacI gene about 10-fold." CDS 81..1160 /label=lacI /note="lac repressor" protein_bind 1176..1197 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 1406..1434 /label=tac promoter /note="strong E. coli promoter; hybrid between the trp and lac UV5 promoters" protein_bind 1442..1458 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." CDS 1528..2703 /label=MBP /note="maltose binding protein from E. coli" CDS 2752..2766 /label=enterokinase site /note="enterokinase recognition and cleavage site" misc_feature 2766..2840 /label=MCS /note="MCS" /note="multiple cloning site" terminator 2841..2927 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 3019..3046 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" promoter 3073..3164 /label=AmpR promoter CDS 3165..4022 /label=AmpR /note="beta-lactamase" rep_origin 4113..4701 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5074..5262) /label=rop /note="Rop protein, which maintains plasmids at low copy number"
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