Basic Vector Information
- Vector Name:
- pET-30c(+)
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5423 bp
- Type:
- pET & Duet Vectors (Novagen)
- Replication origin:
- ori
- Source/Author:
- Novagen (EMD Millipore)
- Copy Number:
- High copy number
pET-30c(+) vector Map
pET-30c(+) vector Sequence
LOCUS pET-30c(+). 5423 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expression of N-terminally S-tagged proteins with an enterokinase site. For other reading frames, use pET-30a(+) or pET-30b(+). ACCESSION . VERSION . KEYWORDS pET-30c(+) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5423) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 5423) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5423 /mol_type="other DNA" /organism="synthetic DNA construct" terminator complement(26..73) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(140..157) /label=6xHis /note="6xHis affinity tag" misc_feature 158..218 /label=MCS /note="MCS" /note="multiple cloning site" CDS complement(220..234) /label=enterokinase site /note="enterokinase recognition and cleavage site" CDS complement(250..294) /label=S-Tag /note="affinity and epitope tag derived from pancreatic ribonuclease A" CDS complement(301..318) /label=thrombin site /note="thrombin recognition and cleavage site" CDS complement(328..345) /label=6xHis /note="6xHis affinity tag" CDS complement(346..348) /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" RBS complement(356..378) /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(393..417) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(418..436) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 749..826 /label=lacI promoter CDS 827..1906 /label=lacI /note="lac repressor" protein_bind 1922..1943 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS 2718..2906 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 3011..3153 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(3339..3927) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS 4049..4861 /label=KanR /note="aminoglycoside phosphotransferase" rep_origin complement(4957..5412) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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