Basic Vector Information
- Vector Name:
- pET-42c(+)
- Antibiotic Resistance:
- Kanamycin
- Length:
- 5931 bp
- Type:
- pET & Duet Vectors (Novagen)
- Replication origin:
- ori
- Source/Author:
- Novagen (EMD Millipore)
- Copy Number:
- High copy number
pET-42c(+) vector Map
pET-42c(+) vector Sequence
LOCUS pET-42c(+). 5931 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expressing GST fusion proteins with a Factor Xa site. For other reading frames, use pET-42a(+) or pET-42b(+). ACCESSION . VERSION . KEYWORDS pET-42c(+) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5931) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 5931) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5931 /mol_type="other DNA" /organism="synthetic DNA construct" terminator complement(26..73) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(150..173) /label=8xHis /note="8xHis affinity tag" misc_feature 174..265 /label=MCS /note="MCS" /note="multiple cloning site" CDS complement(266..277) /label=Factor Xa site /note="Factor Xa recognition and cleavage site" CDS complement(308..352) /label=S-Tag /note="affinity and epitope tag derived from pancreatic ribonuclease A" CDS complement(368..385) /label=thrombin site /note="thrombin recognition and cleavage site" CDS complement(395..412) /label=6xHis /note="6xHis affinity tag" CDS complement(440..1093) /label=GST /note="glutathione S-transferase from Schistosoma japonicum" RBS complement(1101..1123) /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(1138..1162) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(1163..1181) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 1494..1571 /label=lacI promoter CDS 1572..2651 /label=lacI /note="lac repressor" protein_bind 2667..2688 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS 3226..3414 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 3519..3661 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(3847..4435) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS 4557..5369 /label=KanR /note="aminoglycoside phosphotransferase" rep_origin complement(5465..5920) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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