Basic Vector Information
- Vector Name:
- pET-52b(+)
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5227 bp
- Type:
- pET & Duet Vectors (Novagen)
- Replication origin:
- ori
- Source/Author:
- Novagen (EMD Millipore)
- Copy Number:
- High copy number
pET-52b(+) vector Map
pET-52b(+) vector Sequence
LOCUS pET-52b(+). 5227 bp DNA circular SYN 01-JAN-1980 DEFINITION Bacterial vector for expressing proteins with a cleavable N-terminal Strep-Tag II tag and a cleavable C-terminal 10xHis tag. ACCESSION . VERSION . KEYWORDS pET-52b(+) SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5227) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 5227) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5227 /mol_type="other DNA" /organism="synthetic DNA construct" terminator complement(26..73) /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" CDS complement(113..139) /label=9xHis /note="9xHis affinity tag" CDS complement(146..163) /label=thrombin site /note="thrombin recognition and cleavage site" misc_feature 167..208 /label=MCS /note="MCS" /note="multiple cloning site" CDS complement(209..232) /label=HRV 3C site /note="recognition and cleavage site for human rhinovirus 3C and PreScission proteases" CDS complement(239..262) /label=Strep-Tag II /note="peptide that binds Strep-Tactin(R), an engineered form of streptavidin" CDS complement(269..271) /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" RBS complement(278..300) /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" protein_bind complement(315..339) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(340..358) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" promoter 671..748 /label=lacI promoter CDS 749..1828 /label=lacI /note="lac repressor" protein_bind 1844..1865 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." CDS 2403..2591 /label=rop /note="Rop protein, which maintains plasmids at low copy number" misc_feature 2696..2838 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(3024..3612) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" promoter 3693..3785 /label=AmpR promoter CDS 3786..4643 /label=AmpR /note="beta-lactamase" rep_origin complement(4761..5216) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
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