Basic Vector Information
- Vector Name:
- pPICZ C
- Antibiotic Resistance:
- Bleomycin
- Length:
- 3329 bp
- Type:
- Yeast Plasmids
- Replication origin:
- ori
- Source/Author:
- Invitrogen (Life Technologies)
- Copy Number:
- High copy number
- Promoter:
- AOX1
pPICZ C vector Map
pPICZ C vector Sequence
LOCUS 40924_34580 3329 bp DNA circular SYN 13-SEP-2021 DEFINITION synthetic circular DNA. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3329) TITLE Direct Submission REFERENCE 2 (bases 1 to 3329) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3329 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 2..940 /label=AOX1 promoter /note="inducible promoter, regulated by methanol" CDS 1011..1040 /codon_start=1 /label=Myc /note="Myc (human c-Myc proto-oncogene) epitope tag" /translation="EQKLISEEDL" CDS 1056..1073 /codon_start=1 /label=6xHis /note="6xHis affinity tag" /translation="HHHHHH" terminator 1153..1399 /label=AOX1 terminator /note="transcription terminator for AOX1" promoter 1414..1825 /label=TEF1 promoter /note="promoter for EF-1-alpha" promoter 1833..1880 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 1899..2270 /codon_start=1 /label=BleoR /note="antibiotic-binding protein" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" terminator 2339..2586 /label=CYC1 terminator /note="transcription terminator for CYC1" rep_origin complement(2661..3249) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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