YPD Broth is a commonly used yeast growth media for maintenance and propagation of Pichia pastoris (P. pastoris) and Saccharomyces cerevisae (S. cerevisae) yeast strains.
To prepare L YPD Broth
Table 1. Recipe
| Name | Amount | Concentration | Formula/M.W. | CAS # |
|---|---|---|---|---|
| Peptone | 20 g | 20 g/L | /0 | 73049-73-7 |
| yeast extract | 10 g | 10 g/L | /0 | 8013-01-2 |
| Glucose | 20 g | 20 g/L | C6H12O6/180.16 | 50-99-7 |
- Prepare 0.8 L of H2O in a suitable container.
- Add 20 g of Peptone to the solution.
- Add 10 g of yeast extract to the solution.
- Add 20 g of Glucose to the solution.
- Add H2O until the volume is 1 L.
- Autoclave for 15 min on liquid cycle.