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Tris-Buffered Saline (TBS) 10× Preparation and Recipe

Tris-buffered saline (TBS) is an excellent wash buffer for many types of immunoassays.

To prepare L  Tris-Buffered Saline (TBS) 10×

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Tris base24.228 g0.2 MC4H11NO3/121.1477-86-1
Sodium chloride87.66 g1.5 MNaCl/58.447647-14-5
  1. Prepare 0.8 L of H2O in a suitable container.
  2. Add 24.228 g of Tris base to the solution.
  3. Add 87.66 g of Sodium chloride to the solution.
  4. Add H2O until the volume is 1 L.
  5. Adjust pH to 7.6 with 12 N HCl before adjusting to final volume with water. Prepared TBS is stable at 4 °C for 3 months.