Streptomycin Stock Solution Preparation and Recipe

To prepare L  Streptomycin Stock Solution

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Streptomycin sulfate100 g100 mg/mLC42H84N14O36S3/1457.383810-74-0
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 100 g of Streptomycin sulfate to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Prewet a 0.22 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water. Sterilize streptomycin stock through the prepared 0.22 µm syringe filter. Stock may be kept at -20°C for 1 year. Working Conc. = 100 µg/mL