SOC is often used during the transformation of E. coli competent cells with plasmids (added in transformed cells after heat shock/electroporation). SOC = SOB+Glucose
To prepare L SOC Medium
Table 1. Recipe
| Name | Amount | Concentration | Formula/M.W. | CAS # |
|---|---|---|---|---|
| tryptone | 20 g | 20 g/L | /0 | 75-75-2 |
| yeast extract | 5 g | 5 g/L | /0 | 8013-01-2 |
| Sodium chloride | 0.5 g | 0.5 g/L | NaCl/58.44 | 7647-14-5 |
| Potassium Chloride | 0.186 g | 0.186 g/L | KCl/74.55 | 7447-40-7 |
- Prepare 0.8 L of H2O in a suitable container.
- Add 20 g of tryptone to the solution.
- Add 5 g of yeast extract to the solution.
- Add 0.5 g of Sodium chloride to the solution.
- Add 0.186 g of Potassium Chloride to the solution.
- Adjust pH with NaOH solution to 7.0
- Add H2O until the volume is 1 L.
- After autoclaving add 20ml filtered 1M Glucose(cas: 50-99-7) and 5ml autoclaved 2M Magnesium chloride.