SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe

To prepare L  SDS-PAGE SDS Running Buffer (10x)

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Tris base30.285 g0.25 MC4H11NO3/121.1477-86-1
Glycine144.4 g1.923 MNH2CH2COOH/75.0756-40-6
Sodium dodecyl sulfate (SDS)10 g1% w/vC12H25O4NaS/288.38151-21-3
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 30.285 g of Tris base to the solution.
  3. Add 144.4 g of Glycine to the solution.
  4. Add 10 g of Sodium dodecyl sulfate (SDS) to the solution.
  5. Add distilled water until the volume is 1 L.