Pyronin Y (PY) Solution Preparation and Recipe

Pyronin Y(G) (PY) is a dye used for DNA staining. PY has orange spectral emission that is easily distinguishable from FITC and 7-AAD.

To prepare L  Pyronin Y (PY) Solution

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Pyronin Y1 g3.302 mMC17H19N2OCl/302.8192-32-0
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 1 g of Pyronin Y to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Store at 4°C protected from light.