Designed for increased replication of phage Lambda. To encourage multi phage insertion into the host cell, it is recommended that 0.2% maltose be added (prepare a 20% solution and add 1ml per 100ml of medium), which promotes expression of LamB (lambda receptor). If maltose is added, the medium should not be used to create phage stocks, as binding of phage particles to membrane fragments will occur because of increased LamB density.
To prepare L NZYM Broth (NZY)
Table 1. Recipe
| Name | Amount | Concentration | Formula/M.W. | CAS # |
|---|---|---|---|---|
| NZ Amine A | 10 g | 10 g/L | /0 | 91079-40-2 |
| 0.976 g | 8.114 mM | MgSO4/120.37 | 7487-88-9 | |
| Sodium chloride | 5 g | 5 g/L | NaCl/58.44 | 7647-14-5 |
| yeast extract | 5 g | 5 g/L | /0 | 8013-01-2 |
- Prepare 0.8 L of H2O in a suitable container.
- Add 10 g of NZ Amine A to the solution.
- Add 0.976 g of Magnesium sulfate anhydrous to the solution.
- Add 5 g of Sodium chloride to the solution.
- Add 5 g of yeast extract to the solution.
- Add H2O until the volume is 1 L.
- Adjust pH with NaOH sol. to 7.0. Autoclave for 15 min on liquid cycle.