NZM Broth Preparation and Recipe

To prepare L  NZM Broth

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
NZ Amine A10 g10 g/L/091079-40-2
0.976 g8.114 mMMgSO4/120.377487-88-9
Sodium chloride5 g5 g/LNaCl/58.447647-14-5
  1. Prepare 0.8 L of H2O in a suitable container.
  2. Add 10 g of NZ Amine A to the solution.
  3. Add 0.976 g of Magnesium sulfate anhydrous to the solution.
  4. Add 5 g of Sodium chloride to the solution.
  5. Add H2O until the volume is 1 L.
  6. Adjust pH with NaOH sol. to 7.0