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Gey's Balanced Salt Solution Preparation and Recipe

Gey's Balanced Salt Solution can be used for cell suspension or as washing solution.

To prepare L  Gey's Balanced Salt Solution

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
0.166 g1.496 mMCaCl2/110.9810043-52-4
Potassium Chloride0.37 g4.963 mMKCl/74.557447-40-7
Potassium dihydrogen phosphate0.03 g0.22 mMKH2PO4/136.097778-77-0
0.098 g1.032 mMMgCl2/95.217786-30-3
0.034 g0.284 mMMgSO4/120.377487-88-9
Sodium chloride7.999 g0.136 MNaCl/58.447647-14-5
Sodium bicarbonate0.226 g2.702 mMNaHCO3/84.01144-55-8
0.12 g0.845 mMNa2HPO4/141.967558-79-4
Glucose1 g5.55 mMC6H12O6/180.1650-99-7
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 0.166 g of Calcium Chloride to the solution.
  3. Add 0.37 g of Potassium Chloride to the solution.
  4. Add 0.03 g of Potassium dihydrogen phosphate to the solution.
  5. Add 0.098 g of Magnesium chloride to the solution.
  6. Add 0.034 g of Magnesium sulfate anhydrous to the solution.
  7. Add 7.999 g of Sodium chloride to the solution.
  8. Add 0.226 g of Sodium bicarbonate to the solution.
  9. Add 0.12 g of Sodium phosphate dibasic to the solution.
  10. Add 1 g of Glucose to the solution.
  11. Add distilled water until the volume is 1 L.
  12. Sterile filter and store in 500-mL aliquots at 4°C.