Gentamicin Stock Solution Preparation and Recipe

To prepare L  Gentamicin Stock Solution

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Gentamicin20 g20 mg/mLC21H43N5O7/477.61403-66-3
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 20 g of Gentamicin to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Prewet a 0.22 µm syringe filter by drawing through 5-10 ml of sterile H2O and discard water. Sterilize Gentamicin Stock through the prepared 0.22 µm syringe filter. Stock may be kept at -20°C for 1 year. Working Conc. = 20 µg/mL