Ethidium Bromide Solution Preparation and Recipe

To prepare L  Ethidium Bromide Solution

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Ethidium bromide10 g0.025 MC21H20BrN3/394.321239-45-8
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 10 g of Ethidium bromide to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Stir on a magnetic stirrer for several hours to ensure that the dye has dissolved. Wrap the container in aluminum foil or transfer the 10 mg/mL solution to a dark bottle and store at room temperature.