Electrophoretic Transfer Buffer (20x) Preparation and Recipe

To prepare L  Electrophoretic Transfer Buffer (20x)

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
35.292 g0.12 MC6H5Na3O7·2H2O/294.16132-04-3
22.713 g0.16 MNa2HPO4/141.967558-79-4
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 35.292 g of Sodium citrate dihydrate to the solution.
  3. Add 22.713 g of Sodium phosphate dibasic to the solution.
  4. Add distilled water until the volume is 1 L.