Electrode Buffer Preparation and Recipe

To prepare L  Electrode Buffer

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Tris base11.629 g0.096 MC4H11NO3/121.1477-86-1
Glycine37.535 g0.5 MNH2CH2COOH/75.0756-40-6
Sodium dodecyl sulfate (SDS)4 g0.4% w/vC12H25O4NaS/288.38151-21-3
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 11.629 g of Tris base to the solution.
  3. Add 37.535 g of Glycine to the solution.
  4. Add 4 g of Sodium dodecyl sulfate (SDS) to the solution.
  5. Add distilled water until the volume is 1 L.