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Citrate Buffer (pH 3.0 to 6.2) Preparation and Recipe

Citrate buffers can be used for RNA isolation, due to its ability to prevent base hydrolysis. The buffer is also used for antigen detection by breaking cross-links between antigens and any substances in its fixation medium. Citrate buffer is a popular choice for immunofluorescent staining applications, as it can aid in stain intensity without contributing to background signal. It is a popular choice for biological research and clinical applications, including pathology, and has had an increasing role in the medical field, as it has been shown to mildly improve dialysis efficiency.

To prepare L  Citrate Buffer (pH 3.0 to 6.2)

M

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
7.671 g0.039 MC6H8O7/192.1277-92-9
15.501 g0.06 MC6H5O7Na3/258.0768-04-2
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 7.671 g of Citric acid to the solution.
  3. Add 15.501 g of tri-Sodium citrate to the solution.
  4. Add distilled water until the volume is 1 L.
  5. It has a shelf life of up to 3 months at room temperature.