Anesthetic (MS222) Solution Preparation and Recipe

Anesthetic Solution (Buffered MS222) is commonly used to temporarily immobilize fish and amphibians.

To prepare L  Anesthetic (MS222) Solution

Table 1. Recipe

1. Prepare 0.8 L of distilled water in a suitable container.
2. Add 1 g of MS222(3-aminobenzoic acid ethyl ester) to the solution.
3. Add 0.7 g of Sodium bicarbonate to the solution.
4. Add distilled water until the volume is 1 L.
5. The resulting pH should be ∼7.0. Store in a foil-covered container at 4°C for up to 1 month. Warm to room temperature before use.