Ampicillin Stock Solution Preparation and Recipe

To prepare L  Ampicillin Stock Solution

Table 1. Recipe

1. Prepare 0.8 L of distilled water in a suitable container.
2. Add 100 g of Ampicillin Na to the solution.
3. Add distilled water until the volume is 1 L.
4. If sterilization is required, prewash a 0.45- or 0.22-μm sterile filter by drawing through 50-100 mL of H2O. Then pass the ampicillin solution through the washed filter. Store the ampicillin in aliquots at -20°C for 1 yr (or at 4°C for 3 mo). In general, use carbenicillin for ampicillin selection, because it is more chemically stable. Working Conc. = 50-100 µg/mL