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10x TB (Tris-Borate) electrophoresis buffer Preparation and Recipe

To prepare L  10x TB (Tris-Borate) electrophoresis buffer

Table 1. Recipe

NameAmountConcentrationFormula/M.W.CAS #
Tris base107.814 g0.89 MC4H11NO3/121.1477-86-1
Boric acid55.037 g0.89 MH3BO3/61.8410043-35-3
  1. Prepare 0.8 L of distilled water in a suitable container.
  2. Add 107.814 g of Tris base to the solution.
  3. Add 55.037 g of Boric acid to the solution.
  4. Add distilled water until the volume is 1 L.