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Product Name
ZO-2 antibody
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Description
ZO-2 Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive WB detected in HeLa cells, MCF7 cells, mouse heart tissue. Observed molecular weight by Western-blot: 150kd
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Tested applications
ELISA, WB, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
Tight junction protein 2 antibody; Tight junction protein ZO 2 antibody; TJP2 antibody; X104 antibody; ZO 2 antibody; ZO2 antibody; ZO-2 antibody; Zona OCcludens protein 2 antibody; Zonula OCcludens protein 2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ZO-2 recombinant protein (Accession Number: NM_004817). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117261(TJP2 antibody) at dilution of 1:500
Immunofluorescent analysis of MCF-7 cells using Catalog No:117261(TJP2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
TJP2 represents tight junction protein 2 (zona occludens 2)
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References
- Yang F, Wang Z, Wei X. NLRP3 deficiency ameliorates neurovascular damage in experimental ischemic stroke. Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism. 34(4):660-7. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"