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Product Name
ZNF395 antibody
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Description
ZNF395 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas cancer tissue. Positive WB detected in human brain tissue, HeLa cells, HepG2 cells. Observed molecular weight by Western-blot: 62 kDa, 72 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
DKFZp434K1210 antibody; HD regulating factor 2 antibody; HDBP 2 antibody; HDBP2 antibody; HDRF 2 antibody; Papillomavirus binding factor antibody; PBF antibody; PRF 1 antibody; PRF1 antibody; Si 1 8 14 antibody; zinc finger protein 395 antibody; ZNF395 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ZNF395 recombinant protein (Accession Number: NM_018660). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:117172(ZNF395 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human pancreas cancer using Catalog No:117172(ZNF395 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:117172(ZNF395 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ZNF395 is a transcription factors binding to a 7bp GC rich sequence which resides in triplicate at intervals of 13bp within and proximal to the -20bp direct repeat sequences of the Htt promoter. It can bind to regulatory regions in papillomaviruses (PV) and subsequently called the protein papillomavirus binding factor (PBF) [PMID: 11853404]. It contains conserved regions CR1, CR2 and CR3, a domain rich in serines and prolines and have the potential to form a zinc-finger structure, and the C-terminal CR3 is responsible for DNA-binding [PMID: 17897615].
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