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Product Name
Zinc-alpha-2-glycoprotein antibody
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Description
Zinc-alpha-2-glycoprotein Rabbit Polyclonal antibody. Positive WB detected in PC-3 cells, MCF7 cells. Positive IHC detected in human breast cancer tissue. Positive IF detected in PC-3 cells. Observed molecular weight by Western-blot: 41 kDa or 33 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
AZGP1 antibody; ZA2G antibody; ZAG antibody; Zinc-alpha-2-glycoprotein antibody; Zn alpha 2 glycoprotein antibody; Zn alpha 2 GP antibody; ZNGP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Zinc-alpha-2-glycoprotein recombinant protein (Accession Number: NM_001185). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
PC-3 cells were subjected to SDS PAGE followed by western blot with Catalog No:117058(AZGP1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:117058(AZGP1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of PC-3 cells using Catalog No:117058(AZGP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Zinc-alpha-2-glycoprotein (AZGP1) is a soluble protein normally found in body fluids, functions as a lipid mobilizing factor (PMID: 19188554). It is known to be expressed in the secretory epithelia of the liver, lung, breast, GI tract and sweat glands, sharing significant structural similarity with the class I major histocompatibility complex (MHC) antigens (PMID: 3422450). AZGP1 is involved in carcinogenesis and differentiation. Altered expression of AZGP1 has been reported in breast cancer, prostate cancer and lung adenocarcinoma, hepatocellular carcinoma, pancreatic carcinoma and oral tumors (PMID: 22625427).
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References
- Lu CM, Wu YJ, Chen CC. Identification of low-abundance proteins via fractionation of the urine proteome with weak anion exchange chromatography. Proteome science. 9:17. 2011.
- Huang Y, Li LZ, Zhang CZ. Decreased expression of zinc-alpha2-glycoprotein in hepatocellular carcinoma associates with poor prognosis. Journal of translational medicine. 10:106. 2012.
- Balaz M, Vician M, Janakova Z. Subcutaneous adipose tissue zinc-α2-glycoprotein is associated with adipose tissue and whole-body insulin sensitivity. Obesity (Silver Spring, Md.). 22(8):1821-9. 2014.
- Burdelski C, Kleinhans S, Kluth M. Reduced AZGP1 expression is an independent predictor of early PSA recurrence and associated with ERG-fusion positive and PTEN deleted prostate cancers. International journal of cancer. 138(5):1199-206. 2016.
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