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Product Name
ZFYVE27 antibody
- Documents
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Description
ZFYVE27 Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue. Positive WB detected in mouse thymus tissue, Jurkat cells, mouse brain tissue, mouse testis tissue, rat testis tissue. Observed molecular weight by Western-blot: 50kd
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
PROTRUDIN antibody; RP11 459F3.2 antibody; SPG33 antibody; ZFYVE27 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ZFYVE27 recombinant protein (Accession Number: NM_144588). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
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Validations
mouse thymus tissue were subjected to SDS PAGE followed by western blot with Catalog No:117047(ZFYVE27 antibody) at dilution of 1:500
IP Result of anti-ZFYVE27 (IP:Catalog No:117047, 3ug; Detection:Catalog No:117047 1:500) with mouse testis tissue lysate 4000ug.
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Background
ZFYVE27, also named as Protrudin, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. ZFYVE27 localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. ZFYVE27 is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract.
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References
- Chang J, Lee S, Blackstone C. Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation. Proceedings of the National Academy of Sciences of the United States of America. 110(37):14954-9. 2013.
- Hashimoto Y, Shirane M, Matsuzaki F, Saita S, Ohnishi T, Nakayama KI. Protrudin regulates endoplasmic reticulum morphology and function associated with the pathogenesis of hereditary spastic paraplegia. The Journal of biological chemistry. 289(19):12946-61. 2014.
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