-
Product Name
ZCCHC11 antibody
- Documents
-
Description
ZCCHC11 Rabbit Polyclonal antibody. Positive IF detected in Hela. Positive IHC detected in human brain. Positive WB detected in HeLa cells, human brain tissue. Observed molecular weight by Western-blot: 185kd
-
Tested applications
ELISA, WB, IHC, IF
-
Species reactivity
human; other species not tested.
-
Alternative names
ZCCHC11 antibody; Terminal uridylyltransferase 4 antibody; KIAA0191 antibody; TUTase 4 antibody; PAPD3 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of ZCCHC11 recombinant protein (Accession Number: BC048301). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116933(ZCCHC11 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human brain using Catalog No:116933(ZCCHC11 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:116933(ZCCHC11 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using ZCCHC11 antibody Catalog No:116933 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
-
Background
ZCCHC11, also named as KIAA0191 and TUT4, is an uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. ZCCHC11 catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. ZCCHC11 can’t bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. ZCCHC11 may also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"