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Product Name
ZAP70 antibody
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Description
ZAP70 Mouse Monoclonal antibody. Positive WB detected in Jurkat cell, Jurkat cells. Positive IHC detected in human lymphoma tissue. Observed molecular weight by Western-blot: 70 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse; other species not tested.
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Alternative names
70 kDa zeta assOCiated protein antibody; FLJ17670 antibody; FLJ17679 antibody; SRK antibody; STD antibody; Syk related tyrosine kinase antibody; Tyrosine protein kinase ZAP 70 antibody; TZK antibody; ZAP 70 antibody; ZAP70 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of ZAP70 recombinant protein (Accession Number: BC039039). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:107546(ZAP70 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:107546(ZAP70 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:107546(ZAP70 antibody) at dilution of 1:50 (under 40x lens)
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Background
Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.
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