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Product Name
ZAK antibody
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Description
ZAK Rabbit Polyclonal antibody. Positive WB detected in HepG2 cells, human heart tissue, human skeletal muscle tissue, mouse skeletal muscle tissue. Positive IHC detected in human liver tissue, human heart tissue, human placenta tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 55 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
AZK antibody; HCCS 4 antibody; MLK related kinase antibody; MLK7 antibody; mlklak antibody; MLT antibody; MLTK antibody; MRK antibody; ZAK antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ZAK recombinant protein (Accession Number: XM_017004324). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:116903(ZAK antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:116903(ZAK Antibody) at dilution of 1:50(under 10x lens)
Immunohistochemistry of paraffin-embedded human liver tissue slide using Catalog No:116903(ZAK Antibody) at dilution of 1:50(under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:116903(ZAK Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.
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