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Product Name
YY1AP1 antibody
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Description
YY1AP1 Rabbit Polyclonal antibody. Positive WB detected in MDA-MB-231 cells. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 75 kDa and 50 kDa
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Tested applications
ELISA, IF, WB
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Species reactivity
Human; other species not tested.
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Alternative names
FLJ10875 antibody; FLJ13914 antibody; HCCA1 antibody; HCCA2 antibody; YAP antibody; YY1 assOCiated protein 1 antibody; YY1AP antibody; YY1AP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of YY1AP1 recombinant protein (Accession Number: BC003500). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IF: 1:10-1:100
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Validations
MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with Catalog No:116901(YY1AP1 antibody) at dilution of 1:800
Immunofluorescent analysis of HepG2 cells, using YY1AP1 antibody Catalog No:116901 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
YY1AP1 (YY1 associated protein 1), also known as HCCA2 (hepatocellular carcinoma-associated protein 2) or YY1AP, is a 796 amino acid protein that localizes to both the nucleus and the cytoplasm and exists as seven alternatively spliced isoforms. We detected 75 kDa and 50 kDa two isoforms. YY1AP1 is expressed ubiquitously with notable expression in lung, brain, spleen, pancreas, small intestine, heart, colon and skeletal muscle, YY1AP1 interacts with and enhances the transcriptional activation activity of YY1. YY1AP1 is expressed at high levels in liver carcinomas, suggesting a role in tumor transformation and metastasis. YY1AP1 may play a role in cell cycle regulation.
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