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Product Name
YTHDF2 antibody
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Description
YTHDF2 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human testis tissue, human skeletal muscle tissue. Positive WB detected in Jurkat cells, HeLa cells. Positive IP detected in Jurkat cells. Observed molecular weight by Western-blot: 62 kDa
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Tested applications
ELISA, WB, IF, IHC, IP
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Species reactivity
Human; other species not tested.
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Alternative names
CLL assOCiated antigen KW 14 antibody; HGRG8 antibody; NY REN 2 antibody; YTH domain family protein 2 antibody; YTH domain family antibody; member 2 antibody; YTHDF2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of YTHDF2 recombinant protein (Accession Number: NM_016258). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:116895(YTHDF2 Antibody) at dilution of 1:1000
IP Result of anti-YTHDF2 (IP:Catalog No:116895, 4ug; Detection:Catalog No:116895 1:600) with Jurkat cells lysate 2000ug.
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:116895(YTHDF2 Antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HeLa cells using Catalog No:116895(YTHDF2 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
YTHDF2 also named as CLL associated antigen KW 14 or HGRG8 is a 579 amino acid protein, which contains one YTH domain and exists as two alternatively spliced isoforms. YTHDF2 is expressed in in pancreas, testis and placenta. YTHDF2 may play a role in human longevity.
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References
- Zhou J, Wan J, Gao X, Zhang X, Jaffrey SR, Qian SB. Dynamic m(6)A mRNA methylation directs translational control of heat shock response. Nature. 526(7574):591-4. 2015.
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