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Product Name
YTHDF1 antibody
- Documents
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Description
YTHDF1 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, A549 cells, HepG2 cells, Jurkat cells, mouse brain tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human brain tissue, human kidney tissue, human ovary tissue, human spleen tissue, human testis tissue. Observed molecular weight by Western-blot: 61 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
C20orf21 antibody; DACA 1 antibody; FLJ20391 antibody; YTH domain family protein 1 antibody; YTH domain family antibody; member 1 antibody; YTHDF1 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of YTHDF1 recombinant protein (Accession Number: NM_017798). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
WB and IF result of YTHDF1 antibody (Catalog No:116894) with siRNA transfected HeLa cells.
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116894(YTHDF1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human brain using Catalog No:116894(YTHDF1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using Catalog No:116894(YTHDF1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-YTHDF1 (IP:Catalog No:116894, 4ug; Detection:Catalog No:116894 1:1000) with mouse brain tissue lysate 2640ug.
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Background
YTHDF family specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.
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