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Product Name
XPG antibody
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Description
XPG Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive WB detected in HeLa cells, Jurkat cells, K-562 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 200 kDa
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Tested applications
ELISA, WB, IP, IF
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Species reactivity
Human; other species not tested.
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Alternative names
COFS3 antibody; ERCC5 antibody; ERCM2 antibody; UVDR antibody; XPG antibody; XPGC antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of XPG recombinant protein (Accession Number: NM_000123). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116992(ERCC5 antibody) at dilution of 1:500
IP Result of anti-ERCC5 (IP:Catalog No:116992, 4ug; Detection:Catalog No:116992 1:500) with HeLa cells lysate 1200ug.
Immunofluorescent analysis of HeLa cells using Catalog No:116992(ERCC5 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
The human genes correcting DNA repair defects are termed excision-repair cross-complementing or ERCC genes. The ERCC5 gene corrects the excision repair deficiency of Chinese hamster ovary cell line UV135 of complementation group 5. The human ERCC5 gene product is a structure-specific endonuclease required for making the 3-prime incision during DNA nucleotide excision-repair (NER). It also plays an important role in regulating DNA excision repair, removal of bulky lesions caused by environmental chemicals or UV light [PMID:22815677].
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"