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WT1 antibody

Cat.#: 116973

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Product Information

  • Product Name
    WT1 antibody
  • Documents
  • Description
    WT1 Rabbit Polyclonal antibody. Positive WB detected in human kidney tissue, A431 cells, human liver tissue, K-562 cells, MCF7 cells, mouse kidney tissue, rat kidney tissue. Positive IP detected in mouse kidney tissue. Observed molecular weight by Western-blot: 52-55kd
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    GUD antibody; WAGR antibody; Wilms tumor 1 antibody; Wilms tumor protein antibody; WIT 2 antibody; WT1 antibody; WT33 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of WT1 recombinant protein (Accession Number: NM_001198551). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:500-1:5000

  • Validations

    human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:116973(WT1 antibody) at dilution of 1:300

    human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:116973(WT1 antibody) at dilution of 1:300

    IP Result of anti-WT1 (IP:Catalog No:116973, 4ug; Detection:Catalog No:116973 1:1000) with mouse kidney tissue lysate 4000ug.

    IP Result of anti-WT1 (IP:Catalog No:116973, 4ug; Detection:Catalog No:116973 1:1000) with mouse kidney tissue lysate 4000ug.

  • Background
    The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA.
  • References
    • Hua G, Lv X, He C. YAP induces high-grade serous carcinoma in fallopian tube secretory epithelial cells. Oncogene. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"