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Product Name
WRAP53 antibody
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Description
WRAP53 Rabbit Polyclonal antibody. Positive WB detected in human kidney tissue, HeLa cells. Positive IP detected in HeLa cells. Positive IHC detected in human testis tissue, human brain tissue, human heart tissue, human kidney tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue. Observed molecular weight by Western-blot: 72-75kd
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
FLJ10385 antibody; TCAB1 antibody; WDR79 antibody; WRAP53 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of WRAP53 recombinant protein (Accession Number: NM_001143990). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
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Validations
human kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:116839(WRAP53 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human testis using Catalog No:116839(WRAP53 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:116839(WRAP53 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-WRAP53 (IP:Catalog No:116839, 4ug; Detection:Catalog No:116839 1:300) with HeLa cells lysate 2480ug.
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Background
WRAP53 represents WD repeat containing, antisense to TP53
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References
- Rao X, Huang D, Sui X. Overexpression of WRAP53 is associated with development and progression of esophageal squamous cell carcinoma. PloS one. 9(3):e91670. 2014.
- Henriksson S, Rassoolzadeh H, Hedström E. The scaffold protein WRAP53β orchestrates the ubiquitin response critical for DNA double-strand break repair. Genes & development. 28(24):2726-38. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"