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Product Name
WDR61 antibody
- Documents
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Description
WDR61 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human testis tissue, human breast cancer tissue. Positive WB detected in mouse liver tissue, HeLa cells, HepG2 cells, mouse brain tissue, Raji cells. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 33 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
REC14 antibody; SKI8 antibody; WD repeat domain 61 antibody; WDR61 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of WDR61 recombinant protein (Accession Number: NM_025234). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:116814(WDR61 antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human breast cancer slide using Catalog No:116814(WDR61 Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human testis slide using Catalog No:116814(WDR61 Antibody) at dilution of 1:50
IP Result of anti-WDR61 (IP:Catalog No:116814, 4ug; Detection:Catalog No:116814 1:1000) with mouse brain tissue lysate 4000ug.
Immunofluorescent analysis of HeLa cells using Catalog No:116814(WDR61 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency (refer to UniProt). Catalog#22536-1-AP is a rabbit polyclonal antibody raised against the full-length of human WDR61. The MW of this protein is 33 kDa, and this antibody specially recognises the 33 kDa protein.
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