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Product Name
VPS4B antibody
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Description
VPS4B Rabbit Polyclonal antibody. Positive IF detected in A431 cells. Positive WB detected in Y79 cells, A431 cells, HEK-293 cells, HeLa cells, HepG2 cells, MCF7 cells, mouse skin tissue, NIH/3T3 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 50 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
MIG1 antibody; Protein SKD1 antibody; SKD1 antibody; VPS4 2 antibody; VPS42 antibody; VPS4B antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS4B recombinant protein (Accession Number: NM_004869). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IF: 1:10-1:100
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:116792(VPS4B antibody) at dilution of 1:500
IP Result of anti-VPS4B (IP:Catalog No:116792, 4ug; Detection:Catalog No:116792 1:1000) with HeLa cells lysate 880ug.
Immunofluorescent analysis of A431 cells using Catalog No:116792(VPS4B Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
VPS4B(Vacuolar protein sorting-associated protein 4B) is also named as SKD1, VPS42 and belongs to the AAA (ATPases associated with diverse cellular activities) family.It is involved in late steps of the endosomal multivesicular bodies (MVB) pathway and recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission.
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