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Product Name
VPS37A antibody
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Description
VPS37A Rabbit Polyclonal antibody. Positive WB detected in human liver tissue, HeLa cells, MCF-7 cells, mouse liver tissue. Positive IHC detected in human breast cancer tissue. Observed molecular weight by Western-blot: 45 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ESCRT I complex subunit VPS37A antibody; FLJ32642 antibody; FLJ42616 antibody; HCRP1 antibody; hVps37A antibody; PQBP2 antibody; VPS37A antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS37A recombinant protein (Accession Number: XM_017013026). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:116785(VPS37A antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human breast cancer using Catalog No:116785(VPS37A antibody) at dilution of 1:50 (under 10x lens)
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Background
VPS37A, also named as HCRP1, belongs to the VPS37 family. It is a regulator of vesicular trafficking process. VPS37A is a member of the endosomal sorting complex required for transport (ESCRT) system, in upper motor neuron disease. ESCRT has been shown to play a central role in intracellular trafficking, in the maturation of multivesicular bodies and the sorting of ubiquitinated membrane proteins into internal luminal vesicles.
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References
- Agromayor M, Soler N, Caballe A. The UBAP1 subunit of ESCRT-I interacts with ubiquitin via a SOUBA domain. Structure (London, England : 1993). 20(3):414-28. 2012.
- Chen F, Deng J, Liu X, Li W, Zheng J. HCRP-1 regulates cell migration and invasion via EGFR-ERK mediated up-regulation of MMP-2 with prognostic significance in human renal cell carcinoma. Scientific reports. 5:13470. 2015.
- Zivony-Elboum Y, Westbroek W, Kfir N. A founder mutation in Vps37A causes autosomal recessive complex hereditary spastic paraparesis. Journal of medical genetics. 49(7):462-72. 2012.
- Stefani F, Zhang L, Taylor S. UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. Current biology : CB. 21(14):1245-50. 2011.
- Wunderley L, Brownhill K, Stefani F, Tabernero L, Woodman P. The molecular basis for selective assembly of the UBAP1-containing endosome-specific ESCRT-I complex. Journal of cell science. 127(Pt 3):663-72. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"