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Product Name
VPS24 antibody
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Description
VPS24 Rabbit Polyclonal antibody. Positive IHC detected in human heart tissue, human liver tissue, human ovary tissue, human placenta tissue, human skin tissue. Positive IF detected in HepG2 cells. Positive WB detected in HEK-293 cells, human kidney tissue. Positive IP detected in HEK-293 cells. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
CGI 149 antibody; CHMP3 antibody; Chromatin modifying protein 3 antibody; hVps24 antibody; NEDF antibody; VPS24 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS24 recombinant protein (Accession Number: NM_016079). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:116775(VPS24 antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human heart using Catalog No:116775(VPS24 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human heart using Catalog No:116775(VPS24 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using VPS24 antibody Catalog No:116775 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-VPS24 (IP:Catalog No:116775, 4ug; Detection:Catalog No:116775 1:500) with HEK-293 cells lysate 1480ug.
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Background
VPS24 represents vacuolar protein sorting 24 homolog (S. cerevisiae)
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