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Product Name
VPS18 antibody
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Description
VPS18 Rabbit Polyclonal antibody. Positive IHC detected in human lung cancer tissue. Positive IF detected in A549 cells. Positive FC detected in K-562 cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 110 kDa
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Tested applications
ELISA, IP, IHC, FC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
hVPS18 antibody; KIAA1475 antibody; PEP3 antibody; VPS18 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VPS18 recombinant protein (Accession Number: BC001513). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
IP: 1:200-1:1000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
IP Result of anti-VPS18 (IP:Catalog No:116774, 4ug; Detection:Catalog No:116774 1:300) with HeLa cells lysate 920ug.
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:116774(VPS18 antibody) at dilution of 1:100 (under 10x lens)
Immunofluorescent analysis of A549 cells using Catalog No:116774(VPS18 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
1X10^6 K-562 cells were stained with 0.2ug VPS18 antibody (Catalog No:116774, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
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References
- Ulbricht A, Eppler FJ, Tapia VE. Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy. Current biology : CB. 23(5):430-5. 2013.
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