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Product Name
VCP antibody
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Description
VCP Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue, human colon cancer tissue, human lung cancer tissue. Positive IF detected in SH-SY5Y cells. Positive IP detected in HeLa cells. Positive WB detected in SH-SY5Y cells, HeLa cells, human brain tissue, MCF7 cells, mouse brain tissue, mouse lung tissue, mouse thymus tissue, rat brain tissue, rat lung tissue, RAW264.7 cells. Observed molecular weight by Western-blot: 90-100 kDa
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Tested applications
ELISA, WB, IP, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
15S Mg(2+) ATPase p97 subunit antibody; IBMPFD antibody; p97 antibody; TER ATPase antibody; TERA antibody; valosin containing protein antibody; VCP antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VCP recombinant protein (Accession Number: BC007562). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with Catalog No:116726(VCP antibody) at dilution of 1:1000
IP Result of anti-VCP (IP:Catalog No:116726, 4ug; Detection:Catalog No:116726 1:1000) with HeLa cells lysate 1505ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116726(VCP antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:116726(VCP antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of SH-SY5Y cells using Catalog No:116726(VCP Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
VCP(Valosin-containing protein) belongs to the AAA ATPase family.VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975).Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14).
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References
- Tao Z, Wang H, Xia Q. Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity. Human molecular genetics. 24(9):2426-41. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"