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Product Name
VCAM-1 antibody
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Description
VCAM-1 Mouse Monoclonal antibody. Positive IHC detected in human lung cancer tissue. Positive WB detected in human spleen tissue. Observed molecular weight by Western-blot: 110 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
CD106 antibody; DKFZp779G2333 antibody; INCAM 100 antibody; L1CAM antibody; V CAM 1 antibody; VCAM 1 antibody; VCAM1 antibody; VCAM-1 antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of VCAM-1 recombinant protein (Accession Number: NM_001078). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:50-1:500
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Validations
human spleen tissue were subjected to SDS PAGE followed by western blot with Catalog No:107537(VCAM-1 Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using (VCAM-1 Antibody) at dilution of 1:800 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using (VCAM-1 Antibody) at dilution of 1:800 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).
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Background
Vascular cell adhesion molecule 1 (VCAM1), also known as CD106, is a transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. VCAM1 is expressed by cytokine-activated endothelium, interacts with integrin VLA4 (α4β1) present on the surface of leukocytes, and mediates both adhesion and signal transduction. It is also expressed either constitutively or inducibly in a variety of other cell types, including vascular smooth muscle cells, differentiating skeletal muscle cells, renal and neural epithelial cells, macrophages (Kupffer cells), dendritic cells, and bone marrow stromal cells (PMID: 7507076, 11359843).
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