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Product Name
VAV2 antibody
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Description
VAV2 Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human pancreas tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, A431 cells, HEK-293 cells, mouse brain tissue, mouse liver tissue, NIH/3T3 cells, SH-SY5Y cells. Observed molecular weight by Western-blot: 101 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
VAV 2 antibody; VAV2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VAV2 recombinant protein (Accession Number: NM_003371). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116722(VAV2 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:116722(VAV2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:116722(VAV2 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-VAV2 (IP:Catalog No:116722, 4ug; Detection:Catalog No:116722 1:1000) with HeLa cells lysate 2500ug.
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:116722(VAV2 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
VAV2 represents vav 2 guanine nucleotide exchange factor
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"