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Product Name
VARS2 antibody
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Description
VARS2 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, human brain tissue, human liver tissue, Jurkat cells. Observed molecular weight by Western-blot: 118 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
KIAA1885 antibody; Valine tRNA ligase antibody; ValRS antibody; Valyl tRNA synthetase like antibody; VARS2 antibody; VARS2L antibody; VARSL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of VARS2 recombinant protein (Accession Number: BC008844). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:116716(VARS2 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:116716(VARS2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:116716(VARS2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-VARS2 (IP:Catalog No:116716, 4ug; Detection:Catalog No:116716 1:500) with HeLa cells lysate 2400ug.
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Background
VARS2(Valine--tRNA ligase, mitochondrial) is also named as KIAA1885, VARS2L, VARSL and belongs to the class-I aminoacyl-tRNA synthetase family. The deduced 993-amino acid protein has an N-terminal mitochondrial targeting signal with a predicted cleavage site after residue 29. VARS2 has characteristics of a class I mitochondrial aminoacyl-tRNA synthetase, including a classical Rossmann fold.
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References
- Perli E, Giordano C, Pisano A. The isolated carboxy-terminal domain of human mitochondrial leucyl-tRNA synthetase rescues the pathological phenotype of mitochondrial tRNA mutations in human cells. EMBO molecular medicine. 6(2):169-82. 2014.
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