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Product Name
VAMP7/TI-VAMP antibody
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Description
VAMP7/TI-VAMP Rabbit Polyclonal antibody. Positive WB detected in mouse brain tissue, mouse liver tissue. Positive IP detected in mouse brain tissue. Positive IHC detected in human liver tissue, human small intestine tissue. Positive IF detected in HepG2 cells. Observed molecular weight by Western-blot: 20-25 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
SYBL1 antibody; Synaptobrevin like protein 1 antibody; Tetanus insensitive VAMP antibody; TI VAMP antibody; TI-VAMP antibody; VAMP 7 antibody; VAMP7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_005638). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:116709(VAMP7 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:116709(VAMP7 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:116709(VAMP7 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:116709(VAMP7 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
IP Result of anti-VAMP7 (IP:Catalog No:116709, 4ug; Detection:Catalog No:116709 1:1000) with mouse brain tissue lysate 3440ug.
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Background
VAMP7, also named as SYBL1 and TI-VAMP, belongs to the synaptobrevin family. It is involved in the targeting and/or fusion of transport vesicles to their target membrane during transport of proteins from the early endosome to the lysosome. VAMP7 is required for heterotypic fusion of late endosomes with lysosomes and homotypic lysosomal fusion. It is required for calcium regulated lysosomal exocytosis. VAMP7 is involved in the export of chylomicrons from the endoplasmic reticulum to the cis Golgi. It is required for exocytosis of mediators during eosinophil and neutrophil degranulation, and target cell killing by natural killer cells. It is also required for focal exocytosis of late endocytic vesicles during phagosome formation. The antibody is specific to VAMP7.
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